DNA Fingerprinting Activity

Restriction enzyme digests and gel electrophoresis are used for restriction length polymorphism (RFLP) analyses to match DNA samples.

<a href="./resources/rflplesson.doc">Suggested unit lesson plan</a></blockquote></font><br>

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<b>Protocols</b>

 

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<a href="./protocols/reprep.doc">Restriction enzyme digests</a> - protocol for digesting DNA samples with restriction enzymes.  This produces the DNA fragments for fingerprinting.

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<a href="./protocols/gelprep.doc">Gel preparation</a> - protocol for casting agarose gels for electrophoresis.<br><br>

 

<a href="./protocols/rungel.doc">Running a gel</a> - protocol for preparing samples, loading wells, running electrophoresis, and staining for visualization.<br><br>

 

<a href="./protocols/dnastructurelab.doc">DNA structure lab protocol</a> - this is included in the suggested unit lesson plan.<br><br>

 

<a href="./protocols/resafety.doc">Health and safety information for restriction digest protocols</a><br><br>

 

<a href="./protocols/gelsafety.doc">Health and safety information for gel electrophoresis protocols</a><br><br>

 

<a href="./protocols/fishingstory.doc">Fishing story</a> - potential wrap-around story for the RFLP analyses.<br><br>

 

<a href="./protocols/poodlestory.doc">Poodle story</a> - potential wrap-around story for the RFLP analyses.<br><br>

 

<a href="./protocols/pearlstory.doc">Pearl story</a> - potential wrap-around story for the RFLP analyses.<br><br>

 

 

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<b>Instructional videos and presentations</b>

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How to pour, run, and analyze gel electrophoresis:  <a href="http://webfiles.wichita.edu/mschneegurt/gel5-2/gel5-2.html">Get narrated powerpoint now</a><p>

 

How to run restriction enzyme digests:  <a href="http://webfiles.wichita.edu/mschneegurt/rev3/rev3.html">Get narrated powerpoint now</a><p>

 

Instructional powerpoint suitable for students on loading a gel:  <a href="http://webfiles.wichita.edu/mschneegurt/loadgel/loadgel.html">Get powerpoint now</a><p>

 

Student lab protocol handout for restriction digests:  <a href="./resources/studentre.doc">Get it now</a><p>

 

Student lab protocol handout for gel elctrophoresis:  <a href="./resources/studentgel.doc">Get it now</a><p>

 

Background powerpoint on gel electrophoresis:  <a href="http://webfiles.wichita.edu/mschneegurt/gels/gels.html">Get narrated powerpoint now</a><p>

 

Background powerpoint on restriction enzymes 1:  <a href="http://webfiles.wichita.edu/mschneegurt/restrictionenz/restrictionenz.html">Get narrated powerpoint now</a><p>

 

Background powerpoint on restriction enzymes 2:  <a href="http://webfiles.wichita.edu/mschneegurt/restrictionenz2/restrictionenz2.ppt.html">Get narrated powerpoint now</a><p>

 

Background powerpoint on molecular cloning:  <a href="http://webfiles.wichita.edu/mschneegurt/moleccloning/molec-cloning.html">Get narrated powerpoint now</a><p>

 

Background powerpoint on DNA fingerprinting:  <a href="http://webfiles.wichita.edu/mschneegurt/fingerprinting/fingerprinting.html">Get narrated powerpoint now</a><p>